Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Dioscin and Diosgenin on Plateau Hyperuricemia by Attenuating Renal Inflammation via EPHX2

doi: 10.3390/ijms252413399

Figure Lengend Snippet: DIO improves renal indices and serum biochemical indices in rats. ( A ) Body weight. ( B ) Renal body mass index. ( C ) BUN. ( D ) CREA. ( E ) UA. ( F ) TG. **** p < 0.0001, * p < 0.05 vs. control group; #### p < 0.0001, ### p < 0.001, ## p < 0.01, # p < 0.05 vs. model group. data are expressed as mean ± standard deviation (SD), n = 5. Con: Control group; Mod: model group; Allopurino: allopurinol group; DIO-L, M, H: Dio low-, medium-, and high-dose groups. Renal index = kidney weight (g)/body weight (g).

Article Snippet: Sodium carboxymethyl cellulose (CMC-Na) (cat. no. CP300, Shanghai, China) was purchased from Sinopharm Chemical Reagent Co. Allopurinol (cat. no. A21418, Beijing, China) and dimethyl sulfoxide (DMSO) (cat. no. D3855, Beijing, China) was purchased from Beijing InnoChem Science & Technology Co., Ltd. MIRO2 Polyclonal antibody (MIRO2, cat. no.11237-1-Ap, WuHan, China), PCSK9 Polyclonal antibody (PCSK9, cat. no. 27882-1-Ap, Wuhan, China), EPHX2 Polyclonal antibody (EPHX2, cat. no.10833-1-Ap, Wuhan, China), ANGPTL4 Polyclonal antibody (ANGPTL4, cat. no.18374-1-Ap, Wuhan, China), and CPT1A Rabbit PolyAb (CPT1A, cat. no. 15184-1-Ap Wuhan, China) were purchased from ProteinTech.

Techniques: Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Dioscin and Diosgenin on Plateau Hyperuricemia by Attenuating Renal Inflammation via EPHX2

doi: 10.3390/ijms252413399

Figure Lengend Snippet: Effects of DIO on renal histopathology in model rats. ( A ) Hematoxylin–eosin (H&E) staining of kidney (original magnification ×4, scale bar 200 μm; original magnification ×20, scale bar 50 μm). ( B ) Oil red O staining of renal tissues (original magnification ×4, scale bar 200 μm; original magnification ×20, scale bar 50 μm). black arrows in panel ( A ) indicate enlarged glomerular capsule lumen and glomerular atrophy; red arrows indicate enlarged tubular lumen; yellow arrows indicate detachment of the brush border within the proximal tubule. In panel ( B ), the black arrows indicate that the red substances represent lipid droplets. Con: control group; Mod: model group; Allopurino: allopurinol group; DIO-L, M, H: Dio low-, medium-, and high-dose groups.

Article Snippet: Sodium carboxymethyl cellulose (CMC-Na) (cat. no. CP300, Shanghai, China) was purchased from Sinopharm Chemical Reagent Co. Allopurinol (cat. no. A21418, Beijing, China) and dimethyl sulfoxide (DMSO) (cat. no. D3855, Beijing, China) was purchased from Beijing InnoChem Science & Technology Co., Ltd. MIRO2 Polyclonal antibody (MIRO2, cat. no.11237-1-Ap, WuHan, China), PCSK9 Polyclonal antibody (PCSK9, cat. no. 27882-1-Ap, Wuhan, China), EPHX2 Polyclonal antibody (EPHX2, cat. no.10833-1-Ap, Wuhan, China), ANGPTL4 Polyclonal antibody (ANGPTL4, cat. no.18374-1-Ap, Wuhan, China), and CPT1A Rabbit PolyAb (CPT1A, cat. no. 15184-1-Ap Wuhan, China) were purchased from ProteinTech.

Techniques: Histopathology, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Protective Effects of Dioscin and Diosgenin on Plateau Hyperuricemia by Attenuating Renal Inflammation via EPHX2

doi: 10.3390/ijms252413399

Figure Lengend Snippet: Effect of diosgenin on renal inflammatory factors and xanthine oxidase activity. ( A ) 1L-1β. ( B ) 1L-6. ( C ) TNF-α. ( D ) 1L-10. ( E ) Xanthine oxidase activity. **** p < 0.0001, *** p < 0.001, * p < 0.05 vs. control group; #### p < 0.0001, # p < 0.05 vs. model group. Data are expressed as mean ± standard deviation (SD), n = 5. Con: control group; Mod: model group; Allopurino: allopurinol group; DIO-L, M, H: DIO low-, medium-, and high-dose groups.

Article Snippet: Sodium carboxymethyl cellulose (CMC-Na) (cat. no. CP300, Shanghai, China) was purchased from Sinopharm Chemical Reagent Co. Allopurinol (cat. no. A21418, Beijing, China) and dimethyl sulfoxide (DMSO) (cat. no. D3855, Beijing, China) was purchased from Beijing InnoChem Science & Technology Co., Ltd. MIRO2 Polyclonal antibody (MIRO2, cat. no.11237-1-Ap, WuHan, China), PCSK9 Polyclonal antibody (PCSK9, cat. no. 27882-1-Ap, Wuhan, China), EPHX2 Polyclonal antibody (EPHX2, cat. no.10833-1-Ap, Wuhan, China), ANGPTL4 Polyclonal antibody (ANGPTL4, cat. no.18374-1-Ap, Wuhan, China), and CPT1A Rabbit PolyAb (CPT1A, cat. no. 15184-1-Ap Wuhan, China) were purchased from ProteinTech.

Techniques: Activity Assay, Control, Standard Deviation

Journal: Computational and Structural Biotechnology Journal

Article Title: GAPDH: A common housekeeping gene with an oncogenic role in pan-cancer

doi: 10.1016/j.csbj.2023.07.034

Figure Lengend Snippet: GAPDH is regulated by copy number amplification and DNA methylation. (A) DNA copy number variation analysis in 33 cancer types. (B) DNA methylation analysis in 33 cancer types. A red ID probe indicates that the probe targeted to the promotor region. (C) Methylation-specific PCR analysis of the GAPDH promoter in H1299, MDA-MB-231 and T24 cells. (D) mRNA and (E) protein expression of GAPDH in H1299, MDA-MB-231 and T24 cells treated with 5-aza-2'-deoxycytidine. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Human lung adenocarcinoma cell line NCI-H1299 (Cat. no. TCHu160), bladder cancer cell line MDA-MB-231 (Cat. no. TCHu227) and bladder cancer cell line T24 (Cat. no. TCHu 55) were obtained from China Center for Type Culture Collection (China).

Techniques: Amplification, DNA Methylation Assay, Methylation, Expressing